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Methods & Materials

Here's the Step by Step Procedure for this Study

1) Epoxy casts of canine teeth samples excavated from the Himeran Necropolis are examined under a Leica dissecting microscope and photographs are taken of the perikymata using Leica software.

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2) An x-y micro-manipulator is used to examine the entirety of the tooth and its perikymata under the dissecting microscope

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3) Measurements are taken of the individual perikymata on the Leica software and entered into an Excel spreadsheet.

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4) The data from each tooth is then analyzed in excel to identify the number of LEH and the age at which the first defect occurred.

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5) Chi-squared and t-test analysis is performed to determine if any significant correlation exists between LEH and set variables (age, sex, burial style and necropolis). 

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Full Setup

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